Regulatory
Part:BBa_K3054004:Design
Designed by: Dharmik Hitesh Patel Group: iGEM19_Chalmers-Gothenburg (2019-10-20)
pTEF1/pCIT2 dual promoter (yeast)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 1427
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 1427
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 1427
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 1427
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 241
Design Notes
Single promoters for pCIT2 and pTEF1 were extracted from genome using PCR amplification. They were then attached to each other by fusion PCR using linker as complementary sequence for fusion
Source
Saccharomyces cerevisiae genome