DNA

Part:BBa_K3053069

Designed by: Rajeeva Lokshanan   Group: iGEM19_VIT_Vellore   (2019-10-16)


Reverse Complement

This part regulates our system when it is present in a non antibiotic medium where Cro is not expressed.

Usage and Biology

The part is designed in such a way that it is complementary to the RBS (BBa_K3503001). This way, when no ampicillin resistance gene is present (bla) it forms a double stem loop with the RBS and inhibits the expression of the Cro gene which induces the lysogenic cycle of the phage.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None