DNA
Part:BBa_K3053069
Designed by: Rajeeva Lokshanan Group: iGEM19_VIT_Vellore (2019-10-16)
Reverse Complement
This part regulates our system when it is present in a non antibiotic medium where Cro is not expressed.
Usage and Biology
The part is designed in such a way that it is complementary to the RBS (BBa_K3503001). This way, when no ampicillin resistance gene is present (bla) it forms a double stem loop with the RBS and inhibits the expression of the Cro gene which induces the lysogenic cycle of the phage.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |