Composite
The answer

Part:BBa_K3053042:Design

Designed by: Rajeeva Lokshanan   Group: iGEM19_VIT_Vellore   (2019-10-16)


Beta lactamase mRNA targeting sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 204
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Contains spacer sequences. It is also flanked by cut sites for XbaI and SpeI which are then used to help ligate with the plasmid backbone being used.

Source

Synthetic part

References