Composite

Part:BBa_K3052058:Design

Designed by: Xiaoyun Lu   Group: iGEM19_XJTU-CHINA   (2019-10-16)


CI+Pλ+RXY+ho1-pcyA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1402
    Illegal PstI site found at 1951
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2754
    Illegal NheI site found at 2777
    Illegal PstI site found at 1402
    Illegal PstI site found at 1951
    Illegal NotI site found at 1649
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2174
    Illegal BglII site found at 4314
    Illegal BamHI site found at 1956
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1402
    Illegal PstI site found at 1951
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1402
    Illegal PstI site found at 1951
    Illegal NgoMIV site found at 3867
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part contains a SapI site in order to perform Golden Gate Assembly with another part.


Source

This part was assembled by different parts through Golden Gate Assembly.

References