Generator

Part:BBa_K3052014:Design

Designed by: Ma WenHe   Group: iGEM19_XJTU-CHINA   (2019-10-13)


Ptrc+linalool synthase(RXY)+bacterial terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 456
    Illegal PstI site found at 1005
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 456
    Illegal PstI site found at 1005
    Illegal NotI site found at 703
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1228
    Illegal BamHI site found at 1010
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 456
    Illegal PstI site found at 1005
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 456
    Illegal PstI site found at 1005
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part does not contain lacI so it cannot be directly induced by IPTG. In our design, we transfer this circuit with another circuit producing GPP which contains lacI to make it inducible by IPTG.


Source

This part is acquired from chemical synthesis.

References