Regulatory

Part:BBa_K3046029:Design

Designed by: Philip Srensen   Group: iGEM19_DTU-Denmark   (2019-07-04)


PyrG promoter from Aspergillus fumigatus


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 14
    Illegal XbaI site found at 45
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 14
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 14
    Illegal BamHI site found at 39
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 14
    Illegal XbaI site found at 45
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 14
    Illegal XbaI site found at 45
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The promoter is relatively short for a fungal promoter, which makes it easier to de novo synthesize compared to longer fungal promoters.

Source

Sequence originates from Aspergillus flavus.

References