DNA

Part:BBa_K3046024:Design

Designed by: Philip Srensen   Group: iGEM19_DTU-Denmark   (2019-10-20)


AMA1 sequence for autonomous replication in Aspergillus


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1720
    Illegal EcoRI site found at 3997
    Illegal XbaI site found at 665
    Illegal XbaI site found at 1147
    Illegal XbaI site found at 1915
    Illegal XbaI site found at 3802
    Illegal XbaI site found at 4570
    Illegal XbaI site found at 5052
    Illegal SpeI site found at 82
    Illegal SpeI site found at 93
    Illegal SpeI site found at 457
    Illegal SpeI site found at 589
    Illegal SpeI site found at 713
    Illegal SpeI site found at 820
    Illegal SpeI site found at 831
    Illegal PstI site found at 2864
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1720
    Illegal EcoRI site found at 3997
    Illegal SpeI site found at 82
    Illegal SpeI site found at 93
    Illegal SpeI site found at 457
    Illegal SpeI site found at 589
    Illegal SpeI site found at 713
    Illegal SpeI site found at 820
    Illegal SpeI site found at 831
    Illegal PstI site found at 2864
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1720
    Illegal EcoRI site found at 3997
    Illegal XhoI site found at 2261
    Illegal XhoI site found at 2978
    Illegal XhoI site found at 3456
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1720
    Illegal EcoRI site found at 3997
    Illegal XbaI site found at 665
    Illegal XbaI site found at 1147
    Illegal XbaI site found at 1915
    Illegal XbaI site found at 3802
    Illegal XbaI site found at 4570
    Illegal XbaI site found at 5052
    Illegal SpeI site found at 82
    Illegal SpeI site found at 93
    Illegal SpeI site found at 457
    Illegal SpeI site found at 589
    Illegal SpeI site found at 713
    Illegal SpeI site found at 820
    Illegal SpeI site found at 831
    Illegal PstI site found at 2864
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1720
    Illegal EcoRI site found at 3997
    Illegal XbaI site found at 665
    Illegal XbaI site found at 1147
    Illegal XbaI site found at 1915
    Illegal XbaI site found at 3802
    Illegal XbaI site found at 4570
    Illegal XbaI site found at 5052
    Illegal SpeI site found at 82
    Illegal SpeI site found at 93
    Illegal SpeI site found at 457
    Illegal SpeI site found at 589
    Illegal SpeI site found at 713
    Illegal SpeI site found at 820
    Illegal SpeI site found at 831
    Illegal PstI site found at 2864
    Illegal NgoMIV site found at 2998
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5591
    Illegal BsaI.rc site found at 126


Design Notes

This long sequence of native DNA from Aspergillus nidulans does not comply with any of the iGEM assembly standards (RFC[10]/RFC[1000]). FUrthermore, it is relatively hard to both synthesize and amplify by PCR, due to many repeats in this sequence


Source

The original AMA1 sequence was identified from Aspergillus nidulans by: David Gems, Iain L. Johnstone, A.John Clutterbuck, An autonomously replicating plasmid transforms Aspergillus nidulans at high frequency, Gene, Volume 98, Issue 1, 1991, Pages 61-67.

References