DNA

Part:BBa_K3046024

Designed by: Philip Srensen   Group: iGEM19_DTU-Denmark   (2019-10-20)


AMA1 sequence for autonomous replication in Aspergillus

This sequence is used for creating autonomously replicating plasmids to use for Aspergillus spp.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1720
    Illegal EcoRI site found at 3997
    Illegal XbaI site found at 665
    Illegal XbaI site found at 1147
    Illegal XbaI site found at 1915
    Illegal XbaI site found at 3802
    Illegal XbaI site found at 4570
    Illegal XbaI site found at 5052
    Illegal SpeI site found at 82
    Illegal SpeI site found at 93
    Illegal SpeI site found at 457
    Illegal SpeI site found at 589
    Illegal SpeI site found at 713
    Illegal SpeI site found at 820
    Illegal SpeI site found at 831
    Illegal PstI site found at 2864
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1720
    Illegal EcoRI site found at 3997
    Illegal SpeI site found at 82
    Illegal SpeI site found at 93
    Illegal SpeI site found at 457
    Illegal SpeI site found at 589
    Illegal SpeI site found at 713
    Illegal SpeI site found at 820
    Illegal SpeI site found at 831
    Illegal PstI site found at 2864
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1720
    Illegal EcoRI site found at 3997
    Illegal XhoI site found at 2261
    Illegal XhoI site found at 2978
    Illegal XhoI site found at 3456
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1720
    Illegal EcoRI site found at 3997
    Illegal XbaI site found at 665
    Illegal XbaI site found at 1147
    Illegal XbaI site found at 1915
    Illegal XbaI site found at 3802
    Illegal XbaI site found at 4570
    Illegal XbaI site found at 5052
    Illegal SpeI site found at 82
    Illegal SpeI site found at 93
    Illegal SpeI site found at 457
    Illegal SpeI site found at 589
    Illegal SpeI site found at 713
    Illegal SpeI site found at 820
    Illegal SpeI site found at 831
    Illegal PstI site found at 2864
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1720
    Illegal EcoRI site found at 3997
    Illegal XbaI site found at 665
    Illegal XbaI site found at 1147
    Illegal XbaI site found at 1915
    Illegal XbaI site found at 3802
    Illegal XbaI site found at 4570
    Illegal XbaI site found at 5052
    Illegal SpeI site found at 82
    Illegal SpeI site found at 93
    Illegal SpeI site found at 457
    Illegal SpeI site found at 589
    Illegal SpeI site found at 713
    Illegal SpeI site found at 820
    Illegal SpeI site found at 831
    Illegal PstI site found at 2864
    Illegal NgoMIV site found at 2998
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5591
    Illegal BsaI.rc site found at 126


[edit]
Categories
Parameters
None