Plasmid_Backbone

Part:BBa_K3046023:Design

Designed by: Philip Srensen   Group: iGEM19_DTU-Denmark   (2019-10-21)


pAMA1_Amp_PacI-Nt.BbvCI plasmid backbone


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3078
    Illegal EcoRI site found at 5355
    Illegal XbaI site found at 1257
    Illegal XbaI site found at 2023
    Illegal XbaI site found at 2505
    Illegal XbaI site found at 3273
    Illegal XbaI site found at 5160
    Illegal XbaI site found at 5928
    Illegal XbaI site found at 6410
    Illegal SpeI site found at 1440
    Illegal SpeI site found at 1451
    Illegal SpeI site found at 1815
    Illegal SpeI site found at 1947
    Illegal SpeI site found at 2071
    Illegal SpeI site found at 2178
    Illegal SpeI site found at 2189
    Illegal PstI site found at 4222
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3078
    Illegal EcoRI site found at 5355
    Illegal SpeI site found at 1440
    Illegal SpeI site found at 1451
    Illegal SpeI site found at 1815
    Illegal SpeI site found at 1947
    Illegal SpeI site found at 2071
    Illegal SpeI site found at 2178
    Illegal SpeI site found at 2189
    Illegal PstI site found at 4222
    Illegal NotI site found at 1296
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3078
    Illegal EcoRI site found at 5355
    Illegal BglII site found at 7082
    Illegal XhoI site found at 3619
    Illegal XhoI site found at 4336
    Illegal XhoI site found at 4814
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3078
    Illegal EcoRI site found at 5355
    Illegal XbaI site found at 1257
    Illegal XbaI site found at 2023
    Illegal XbaI site found at 2505
    Illegal XbaI site found at 3273
    Illegal XbaI site found at 5160
    Illegal XbaI site found at 5928
    Illegal XbaI site found at 6410
    Illegal SpeI site found at 1440
    Illegal SpeI site found at 1451
    Illegal SpeI site found at 1815
    Illegal SpeI site found at 1947
    Illegal SpeI site found at 2071
    Illegal SpeI site found at 2178
    Illegal SpeI site found at 2189
    Illegal PstI site found at 4222
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3078
    Illegal EcoRI site found at 5355
    Illegal XbaI site found at 1257
    Illegal XbaI site found at 2023
    Illegal XbaI site found at 2505
    Illegal XbaI site found at 3273
    Illegal XbaI site found at 5160
    Illegal XbaI site found at 5928
    Illegal XbaI site found at 6410
    Illegal SpeI site found at 1440
    Illegal SpeI site found at 1451
    Illegal SpeI site found at 1815
    Illegal SpeI site found at 1947
    Illegal SpeI site found at 2071
    Illegal SpeI site found at 2178
    Illegal SpeI site found at 2189
    Illegal PstI site found at 4222
    Illegal NgoMIV site found at 4356
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 6949
    Illegal BsaI.rc site found at 1484


Design Notes

Inserts can be integrated into the plasmid by using a PacI/Nt.BbvCI USER assembly casette, by having primers of any insert have tails with the following sequences: For fusion to upstream part of the plasmid backbone: 5'GGGTTTAAU 3' For fusion to downstream part of plasmid backbone: 5'GGTCTTAAU 3'


Source

This part has not been published, and was obtained internally in the lab.

References