![](https://parts.igem.org/images/partbypart/icon_plasmid_backbone.png)
Plasmid_Backbone
Part:BBa_K3046023:Design
Designed by: Philip Srensen Group: iGEM19_DTU-Denmark (2019-10-21)
pAMA1_Amp_PacI-Nt.BbvCI plasmid backbone
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3078
Illegal EcoRI site found at 5355
Illegal XbaI site found at 1257
Illegal XbaI site found at 2023
Illegal XbaI site found at 2505
Illegal XbaI site found at 3273
Illegal XbaI site found at 5160
Illegal XbaI site found at 5928
Illegal XbaI site found at 6410
Illegal SpeI site found at 1440
Illegal SpeI site found at 1451
Illegal SpeI site found at 1815
Illegal SpeI site found at 1947
Illegal SpeI site found at 2071
Illegal SpeI site found at 2178
Illegal SpeI site found at 2189
Illegal PstI site found at 4222 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3078
Illegal EcoRI site found at 5355
Illegal SpeI site found at 1440
Illegal SpeI site found at 1451
Illegal SpeI site found at 1815
Illegal SpeI site found at 1947
Illegal SpeI site found at 2071
Illegal SpeI site found at 2178
Illegal SpeI site found at 2189
Illegal PstI site found at 4222
Illegal NotI site found at 1296 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3078
Illegal EcoRI site found at 5355
Illegal BglII site found at 7082
Illegal XhoI site found at 3619
Illegal XhoI site found at 4336
Illegal XhoI site found at 4814 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3078
Illegal EcoRI site found at 5355
Illegal XbaI site found at 1257
Illegal XbaI site found at 2023
Illegal XbaI site found at 2505
Illegal XbaI site found at 3273
Illegal XbaI site found at 5160
Illegal XbaI site found at 5928
Illegal XbaI site found at 6410
Illegal SpeI site found at 1440
Illegal SpeI site found at 1451
Illegal SpeI site found at 1815
Illegal SpeI site found at 1947
Illegal SpeI site found at 2071
Illegal SpeI site found at 2178
Illegal SpeI site found at 2189
Illegal PstI site found at 4222 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3078
Illegal EcoRI site found at 5355
Illegal XbaI site found at 1257
Illegal XbaI site found at 2023
Illegal XbaI site found at 2505
Illegal XbaI site found at 3273
Illegal XbaI site found at 5160
Illegal XbaI site found at 5928
Illegal XbaI site found at 6410
Illegal SpeI site found at 1440
Illegal SpeI site found at 1451
Illegal SpeI site found at 1815
Illegal SpeI site found at 1947
Illegal SpeI site found at 2071
Illegal SpeI site found at 2178
Illegal SpeI site found at 2189
Illegal PstI site found at 4222
Illegal NgoMIV site found at 4356 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 6949
Illegal BsaI.rc site found at 1484
Design Notes
Inserts can be integrated into the plasmid by using a PacI/Nt.BbvCI USER assembly casette, by having primers of any insert have tails with the following sequences: For fusion to upstream part of the plasmid backbone: 5'GGGTTTAAU 3' For fusion to downstream part of plasmid backbone: 5'GGTCTTAAU 3'
Source
This part has not been published, and was obtained internally in the lab.