Coding
Part:BBa_K3044024:Design
Designed by: Catharina Bang Jensen Group: iGEM19_SDU-Denmark (2019-10-10)
Codon optimized Cas9 for expression in E. coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 251
Illegal BglII site found at 1325 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The mutations to activate the catalytically domains RuvC and HNH were made by A10D and A840H mutations, respectively. The point mutations made are c.29C>A, c.30G>T for the RuvC domain and c.2518C>G, c.2519C>A, c.2520G>T for the HNH domain.
These mutations are codon optimized according to a codon frequency table [1]
Source
The mutations made to activate the catalytic domains are A10D and A840H in part BBa_K3044008.