Regulatory

Part:BBa_K3040612:Design

Designed by: TSAI, CHIA-TSEN   Group: iGEM19_NTHU_Taiwan   (2019-10-18)


Two FadR binding sites with pLac promoter regulating downstream RFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 30
    Illegal suffix found in sequence at 129
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 30
    Illegal SpeI site found at 130
    Illegal PstI site found at 144
    Illegal NotI site found at 36
    Illegal NotI site found at 137
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 30
    Illegal BglII site found at 50
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 30
    Illegal suffix found in sequence at 130
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 30
    Illegal XbaI site found at 45
    Illegal SpeI site found at 130
    Illegal PstI site found at 144
    Illegal AgeI site found at 762
    Illegal AgeI site found at 874
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

ZHANG, Fuzhong; CAROTHERS, James M.; KEASLING, Jay D. Design of a dynamic sensor-regulator system for production of chemicals and fuels derived from fatty acids. Nature biotechnology, 2012, 30.4: 354.


Source

ZHANG, Fuzhong; CAROTHERS, James M.; KEASLING, Jay D. Design of a dynamic sensor-regulator system for production of chemicals and fuels derived from fatty acids. Nature biotechnology, 2012, 30.4: 354.

References