Part:BBa_K3040506
pFadBA_NTHU
pFadD promoter is one of the regulator in the enzymes of fatty acid biosynthesis in E. coli. It is composed of two fadR recognition sites, and one CRP binding site. The fatty acid metabolism system of E. coli is consist of many parts, fadD, fadL, fadR, fadA, etc. Interestingly, each enzyme in this family has its own different sequence of fadR recognition site in its promoter, which makes these promoter having different strength, yet can still be interchangeable for us to apply in our system. Based on this fact, we tried pFadD promoter to take place of the weak pFadBA promoter. With two fadR recognition sites native in pFadD, we assume a better result in the sensitivity and a lower leakage in our pFadD promoter.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 65
Illegal suffix found in sequence at 271 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 65
Illegal SpeI site found at 272
Illegal PstI site found at 286
Illegal NotI site found at 71
Illegal NotI site found at 279 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 65
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 65
Illegal suffix found in sequence at 272 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 65
Illegal XbaI site found at 80
Illegal SpeI site found at 272
Illegal PstI site found at 286 - 1000COMPATIBLE WITH RFC[1000]
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