Composite

Part:BBa_K3040503

Designed by: TIEN , TING-LUN   Group: iGEM19_NTHU_Taiwan   (2019-10-14)


Lpp-OmpA-Histag

FadR is one of a regulatory enzyme in fatty acid biosynthesis in E. coli. , which acts as the repressor in the fatty acid metabolism system. In the absence of fatty acids, FadR will attach to the gene fragment, and block the entire gene expression. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

We obtain the sequence of FadR directly by cell disruption of E. coli MG1655. After obtaining FadR, we further combine it with the promoter R0010 and a strong RBS B0030.


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