Composite

Part:BBa_K3040015:Design

Designed by: TSAI, CHIA-TSEN   Group: iGEM19_NTHU_Taiwan   (2019-10-18)


pFadD promoter with one fadR binding sites from pFadBA regulating downstream RFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 33
    Illegal XbaI site found at 219
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 33
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 33
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 33
    Illegal XbaI site found at 219
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 33
    Illegal XbaI site found at 219
    Illegal AgeI site found at 839
    Illegal AgeI site found at 951
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1. FENG, Youjun; CRONAN, John E. Crosstalk of Escherichia coli FadR with global regulators in expression of fatty acid transport genes. PloS one, 2012, 7.9: e46275. 2. ZHANG, Fuzhong; CAROTHERS, James M.; KEASLING, Jay D. Design of a dynamic sensor-regulator system for production of chemicals and fuels derived from fatty acids. Nature biotechnology, 2012, 30.4: 354.



Source

1. FENG, Youjun; CRONAN, John E. Crosstalk of Escherichia coli FadR with global regulators in expression of fatty acid transport genes. PloS one, 2012, 7.9: e46275. 2. ZHANG, Fuzhong; CAROTHERS, James M.; KEASLING, Jay D. Design of a dynamic sensor-regulator system for production of chemicals and fuels derived from fatty acids. Nature biotechnology, 2012, 30.4: 354.


References