Composite

Part:BBa_K3040011:Design

Designed by: TSAI, CHIA-TSEN   Group: iGEM19_NTHU_Taiwan   (2019-10-18)


One FadR binding site with pLac promoter regulating downstream RFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 65
    Illegal suffix found in sequence at 167
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 65
    Illegal SpeI site found at 168
    Illegal PstI site found at 182
    Illegal NotI site found at 71
    Illegal NotI site found at 175
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 65
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 65
    Illegal suffix found in sequence at 168
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 65
    Illegal XbaI site found at 80
    Illegal SpeI site found at 168
    Illegal PstI site found at 182
    Illegal AgeI site found at 833
    Illegal AgeI site found at 945
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

ZHANG, Fuzhong; CAROTHERS, James M.; KEASLING, Jay D. Design of a dynamic sensor-regulator system for production of chemicals and fuels derived from fatty acids. Nature biotechnology, 2012, 30.4: 354.


Source

ZHANG, Fuzhong; CAROTHERS, James M.; KEASLING, Jay D. Design of a dynamic sensor-regulator system for production of chemicals and fuels derived from fatty acids. Nature biotechnology, 2012, 30.4: 354.

References