Regulatory

Part:BBa_K3033000

Designed by: Philip Naderev P.Lagniton   Group: iGEM19_UM_Macau   (2019-10-10)


T7 Promoter

This part is a T7 promoter that is inducible with IPTG. Teams could use this sequence as a regulator for the expression of their desired protein. The T7 promoter is a sequence of DNA 18 base pairs long up to transcription start site at +1 (5’ – TAATACGACTCACTATAG – 3’) that is recognized by T7 RNA polymerase . The T7 promoter is commonly used to regulate gene expression of recombinant proteins, which can be subsequently used for a variety of downstream research applications.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None