Composite
Part:BBa_K3032059:Design
Designed by: Denis Baronas Group: iGEM19_Vilnius-Lithuania (2019-10-15)
sfGFP tagged with light-inducible protein degradation tag (168AtLOV2, Ready for Expression)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1189
Illegal SapI.rc site found at 74
Design Notes
Degradation tag consists of AtLOV2 whose length is 168aa.
Source
BBa_J23100 BBa_B0034 BBa_I746916 BBa_K3032009 BBa_B0015