Composite

Part:BBa_K3032050:Design

Designed by: Denis Baronas   Group: iGEM19_Vilnius-Lithuania   (2019-10-15)


sfGFP tagged with light-inducible protein degradation tag (159AtLOV2, Ready for Expression)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1263
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1189
    Illegal SapI.rc site found at 74


Design Notes

Degradation tag consists of AtLOV2 whose length is 159aa.



Source

BBa_J23100 BBa_B0034 BBa_I746916 BBa_K3032000 BBa_B0015


References