Regulatory
Part:BBa_K3032026:Design
Designed by: Denis Baronas Group: iGEM19_Vilnius-Lithuania (2019-10-14)
strong LexA promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
LexA DNA binding sequence was carefully inserted into strong promoter’s region of -35 to -10 in order not to affect promoter's activity.
Source
This promoter sequence is design by us. LexA strong DNA binding sequence (doi:10.1093/nar/gki939) was inserted into strong promoter’s region of -35 to -10.