Regulatory

Part:BBa_K3032026:Design

Designed by: Denis Baronas   Group: iGEM19_Vilnius-Lithuania   (2019-10-14)


strong LexA promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

LexA DNA binding sequence was carefully inserted into strong promoter’s region of -35 to -10 in order not to affect promoter's activity.



Source

This promoter sequence is design by us. LexA strong DNA binding sequence (doi:10.1093/nar/gki939) was inserted into strong promoter’s region of -35 to -10.



References