Coding

Part:BBa_K3032016:Design

Designed by: Denis Baronas   Group: iGEM19_Vilnius-Lithuania   (2019-10-13)


BphP1 (bacterial phytochrome photoreceptor)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1613
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 669
    Illegal AgeI site found at 2056
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for E. coli.


Source

BphP1 protein sequence was taken from https://www.uniprot.org/uniprot/A0A161I5N6 (Rhodopseudomonas palustris) and codon optimized for E. coli.


References