Tag

Part:BBa_K3032009:Design

Designed by: Denis Baronas   Group: iGEM19_Vilnius-Lithuania   (2019-10-12)


Light-inducible protein degradation tag (168AtLOV2)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 400


Design Notes

LOV2 domain was fused with protein degradation tag of Mesoplasma florum for the new light-inducible protein degradation tag.



Source

LOV2 domain sequence was taken from Arabidopsis thaliana phototropin 1 https://www.ncbi.nlm.nih.gov/protein/NP_001030814.1 and protein degradation tag of Mesoplasma florum (mf-Lon) from https://parts.igem.org/wiki/index.php?title=Part:BBa_K2333001


References