Coding
Part:BBa_K3031018:Design
Designed by: David Doyle Group: iGEM19_SUIS_Shanghai (2019-10-15)
OxyR mutated at residues 147 & 203
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 720
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 673
Design Notes
Mutations were made to the amino acid sequence with the aim of improving the reactivity of the reactive cysteine (Cys-199). See our model for the various data we used to determine the parameters used to help improve this part. The main focus was to increase the solvent accessibility of the cysteine.
Source
Biobrock - mutations made with model.