Composite

Part:BBa_K3027004:Design

Designed by: Arnaud Boudigou   Group: iGEM19_GO_Paris-Saclay   (2019-10-16)


Arabinose-inducible nuclease_A1 expression module


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1216
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1216
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1216
    Illegal BamHI site found at 1144
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1216
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1216
    Illegal AgeI site found at 979
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since we used Golden Gate assembly, we made sure that our parts were devoid of BsaI and SapI sites.


Source

A1 is a nuclease coming from bacteriophage T5.

References