Coding

Part:BBa_K3024000:Design

Designed by: Tobias Willi   Group: iGEM19_Stockholm   (2019-09-24)


C protein of P2 Bacteriophage

This basic part is used in the composite part BBa_K3024004 which can be used to induce lysogeny upon P2 phage infection of E. coli.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Leucin 53 and Glutamin 54 codons were changed so they dont contain Pst1 restriction site to make it RFC 10 compatible.


Source

Enterobacteria phage P2 genome taken from NCBI https://www.ncbi.nlm.nih.gov/nuccore/NC_001895

References

Christie GE, Calendar R. Bacteriophage P2. Bacteriophage. 2016;6(1):e1145782.