Translational_Unit

Part:BBa_K302034:Design

Designed by: Philip Hall   Group: iGEM10_Newcastle   (2010-10-25)

mazEF toxin-antitoxin cluster


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 67
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence selected based on the work of Pellegrini at al. in the paper below. K302032 encodes mazE, K302033 encodes mazF. Coding sequences overlap at base 269, mazF native RBS is incorporated into mazE CDS.

Source

Bacillus Subtilis 168 strain

References

Pellegrini, O., Mathy, N., Gogos, A., Shapiro, L., and Condon, C. (2005) The Bacillus subtilis ydcDE operon encodes an endoribonuclease of the MazF/PemK family and its inhibitor. Mol Microbiol 56: 1139–1148.