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Sample Discontinued
Experience: None
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Coding

Part:BBa_K302025:Design

Designed by: Steven Woodhouse   Group: iGEM10_Newcastle   (2010-10-03)


spaB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 75
    Illegal EcoRI site found at 1141
    Illegal XbaI site found at 1920
    Illegal PstI site found at 2570
    Illegal PstI site found at 2888
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 75
    Illegal EcoRI site found at 1141
    Illegal PstI site found at 2570
    Illegal PstI site found at 2888
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 75
    Illegal EcoRI site found at 1141
    Illegal BamHI site found at 2391
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 75
    Illegal EcoRI site found at 1141
    Illegal XbaI site found at 1920
    Illegal PstI site found at 2570
    Illegal PstI site found at 2888
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 75
    Illegal EcoRI site found at 1141
    Illegal XbaI site found at 1920
    Illegal PstI site found at 2570
    Illegal PstI site found at 2888
    Illegal AgeI site found at 2205
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 257
    Illegal SapI.rc site found at 2305


Design Notes

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Source

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References