Translational_Unit

Part:BBa_K302017

Designed by: Steven Woodhouse, Alan Koh, Rachel May Boyd, Philip Hall   Group: iGEM10_Newcastle   (2010-08-18)

spaIFEG cluster

Coding sequences and RBS' for spaIFEG cluster.

Subtilin is a lantibiotic peptide produced naturally by Bacillus subtilis ATCC 6633 but not by Bacillus subtilis 168.

spaI encodes a membrane bound protein that binds subtilin and prevents it from penetrating the cell membrane to form pores.

spaFEG encode three proteins which form a membrane transporter that removes subtilin from the membrane.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 740
    Illegal BglII site found at 1488
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1755
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1035
    Illegal SapI site found at 1315
    Illegal SapI.rc site found at 2038


[edit]
Categories
Parameters
None