Device
Part:BBa_K302014:Design
Designed by: Alan Koh, Steven Woodhouse, Harsh Sheth Group: iGEM10_Newcastle (2010-08-13)
IPTG-inducible arginase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 267
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Two EcoR1 restriction sites were removed from the rocF coding sequence.
Source
The rocF sequence was taken from Bacillus subtilis 168 genomic DNA, by PCR. Pspac oid,. .... assembled by the Gibson method...