Coding

Part:BBa_K3019015:Design

Designed by: Valeriia Leoshko   Group: iGEM19_Tartu_TUIT   (2019-10-13)


α-factor pre-pro signal 3HA BBa_K2711000


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 472
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 587
    Illegal BamHI site found at 807
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 476
    Illegal NgoMIV site found at 838
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

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Source

BBa_K2711000 sequence is taken from iGEM registry. Alfa factor pre-pro signal sequence was taken from yeast genomic DNA and 3HA tag sequence was kindly provided by professor Mart Loog research group. 3HA tag was placed to pre-pro signal C-terminal. The whole sequence was ordered from IDT.


References