Coding

Part:BBa_K3019014:Design

Designed by: Valeriia Leoshko   Group: iGEM19_Tartu_TUIT   (2019-10-13)


Pre-Ost1-pro-α-factor signal BBa_K2711000


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 395
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 510
    Illegal BamHI site found at 730
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 399
    Illegal NgoMIV site found at 761
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-


Source

BBa_K2711000 sequence is taken from iGEM registry, pre-Ost1-pro-α-factor signal

sequence was taken from yeast genomic DNA.


References