Coding

Part:BBa_K3019007:Design

Designed by: Valeriia Leoshko   Group: iGEM19_Tartu_TUIT   (2019-10-13)


Pre-Ost1-pro-α-factor signal GLCI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 558
    Illegal AgeI site found at 1639
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-


Source

glcI gene is from Arthrobacter sp. YCWD3. The sequence of the part was obtained from NCBI database (https://www.ncbi.nlm.nih.gov/nuccore/D23668.1?report=fasta) and codon optimised for S. Cerevisiae. Pre-Ost1-pro-α-factor signal sequence was taken from yeast genomic DNA.


References