Composite

Part:BBa_K3017069:Design

Designed by: Luk Hau Ching   Group: iGEM19_Hong_Kong_HKUST   (2019-10-17)


dCas9-6XHis


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 852
    Illegal PstI site found at 2274
    Illegal PstI site found at 2478
    Illegal PstI site found at 2508
    Illegal PstI site found at 3720
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 852
    Illegal PstI site found at 2274
    Illegal PstI site found at 2478
    Illegal PstI site found at 2508
    Illegal PstI site found at 3720
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 313
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 852
    Illegal PstI site found at 2274
    Illegal PstI site found at 2478
    Illegal PstI site found at 2508
    Illegal PstI site found at 3720
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 852
    Illegal PstI site found at 2274
    Illegal PstI site found at 2478
    Illegal PstI site found at 2508
    Illegal PstI site found at 3720
    Illegal NgoMIV site found at 1140
    Illegal NgoMIV site found at 2244
    Illegal NgoMIV site found at 2317
    Illegal NgoMIV site found at 2802
    Illegal NgoMIV site found at 3711
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

dCas9 gene is sourced from Streptococcus pyogenes

References