Coding

Part:BBa_K3017011:Design

Designed by: Luk Hau Ching   Group: iGEM19_Hong_Kong_HKUST   (2019-10-14)


dCas9-6XHis


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 790
    Illegal PstI site found at 2212
    Illegal PstI site found at 2416
    Illegal PstI site found at 2446
    Illegal PstI site found at 3658
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 790
    Illegal PstI site found at 2212
    Illegal PstI site found at 2416
    Illegal PstI site found at 2446
    Illegal PstI site found at 3658
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 790
    Illegal PstI site found at 2212
    Illegal PstI site found at 2416
    Illegal PstI site found at 2446
    Illegal PstI site found at 3658
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 790
    Illegal PstI site found at 2212
    Illegal PstI site found at 2416
    Illegal PstI site found at 2446
    Illegal PstI site found at 3658
    Illegal NgoMIV site found at 1078
    Illegal NgoMIV site found at 2182
    Illegal NgoMIV site found at 2255
    Illegal NgoMIV site found at 2740
    Illegal NgoMIV site found at 3649
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Unknown


Design Notes

To be modified


Source

dCas9 gene is sourced from Streptococcus pyogenes

References