Other

Part:BBa_K3009012:Design

Designed by: Carolin Ruckes   Group: iGEM19_Freiburg   (2019-10-15)


Aspartate Racemase (PhAspR)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 363
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For purification purposes, we added a deca-his-tag with a GSG linker at the C-terminus.

Source

Pyrococcus horikoshii

References

A. Ohtaki , Y. Nakano , E. Iizuka , T. Arakawa , K. Yamada and M. Yodha , Proteins, 2008, 70 , 1167 —1174