Coding

Part:BBa_K3008005:Design

Designed by: Shayan Vahdani   Group: iGEM19_UCSC   (2019-10-10)


CAHS 107838


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 150
    Illegal AgeI site found at 625
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Induced silent mutations to remove restriction sites. Changed CAG to CAA to remove PstI site (position 120); Changed TTC to TTT (position 447).


Source

This part comes from the genomic sequence of Paramacrobiotus richtersi.

References