Coding

Part:BBa_K3007025:Design

Designed by: Cheng Li   Group: iGEM19_QHFZ-China   (2019-10-16)


Expression device of HucR & YgfU: Uric acid detector & uric acid transporter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 32
    Illegal EcoRI site found at 1849
    Illegal XbaI site found at 135
    Illegal PstI site found at 38
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 32
    Illegal EcoRI site found at 1849
    Illegal PstI site found at 38
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 32
    Illegal EcoRI site found at 1849
    Illegal BamHI site found at 43
    Illegal BamHI site found at 118
    Illegal BamHI site found at 982
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 32
    Illegal EcoRI site found at 1849
    Illegal XbaI site found at 135
    Illegal PstI site found at 38
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 32
    Illegal EcoRI site found at 1849
    Illegal XbaI site found at 135
    Illegal PstI site found at 38
    Illegal AgeI site found at 1164
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Figure 1. Schematic cartoon of the DNA construct of BBa_K3007025


Source

The key component HucR comes from D. radiodurans, and YgfU comes from E. coli.

References