Coding

Part:BBa_K3007022:Design

Designed by: Cheng Li   Group: iGEM19_QHFZ-China   (2019-10-16)


Expression device of dsRed under PhucR control


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 140
    Illegal PstI site found at 510
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 140
    Illegal PstI site found at 510
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 158
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 140
    Illegal PstI site found at 510
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 140
    Illegal PstI site found at 510
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Figure 1. Schematic cartoon of the DNA construct of BBa_K30070022

Source

The key component of PhucR comes from Deinococcus radiodurans. Others comes from E. coli. We ordered the DNA from a company.

References