Composite

Part:BBa_K3007004:Design

Designed by: Cheng Li   Group: iGEM19_QHFZ-China   (2019-10-15)


Expression device of dsRed under control of uric acid-responsive regulatory system


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1399
    Illegal EcoRI site found at 3216
    Illegal XbaI site found at 3113
    Illegal SpeI site found at 917
    Illegal PstI site found at 140
    Illegal PstI site found at 510
    Illegal PstI site found at 3210
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1399
    Illegal EcoRI site found at 3216
    Illegal NheI site found at 1085
    Illegal SpeI site found at 917
    Illegal PstI site found at 140
    Illegal PstI site found at 510
    Illegal PstI site found at 3210
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1399
    Illegal EcoRI site found at 3216
    Illegal BglII site found at 852
    Illegal BamHI site found at 158
    Illegal BamHI site found at 2266
    Illegal BamHI site found at 3130
    Illegal BamHI site found at 3205
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1399
    Illegal EcoRI site found at 3216
    Illegal XbaI site found at 3113
    Illegal SpeI site found at 917
    Illegal PstI site found at 140
    Illegal PstI site found at 510
    Illegal PstI site found at 3210
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1399
    Illegal EcoRI site found at 3216
    Illegal XbaI site found at 3113
    Illegal SpeI site found at 917
    Illegal PstI site found at 140
    Illegal PstI site found at 510
    Illegal PstI site found at 3210
    Illegal AgeI site found at 2084
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Figure 1. Schematic cartoon of the DNA construct of BBa_K3007004

Same as BBa_K3007029.


Source

HucR and PhucR come from D. radiodurans, while the others come from E. coli.

References