Part:BBa_K3003022

Single Chain Insulin-57 with a linker peptide (Rajpal 27)

We took SCI-57 design from the study “Design of an active ultrastable single-chain insulin analog: synthesis, structure, and therapeutic implications” done by Hua QX et al. The linker we have used between SCI57's b and a chain was Rajpal 27. This linker is taken from the study “Single-Chain Insulins as Receptor Agonists” done by Rajpal G. et al. This linker provides more bioactivity and affinity for single chain insulin to recognized by the receptor rather than Rajpal 15, therefore it increases the affinity of SCI for binding to insulin receptor in a more efficient way.