Coding

Part:BBa_K3003020:Design

Designed by: Aysenur Deniz Cayirtepe   Group: iGEM19_Bilkent-UNAMBG   (2019-10-16)


Single Chain Insulin-57 with a linker peptide


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 115
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 115
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 103
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 115
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 115
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed specifically to examine the compatibility of chains with the linker sequences.


Source

Synthesized by IDT.

References

Chance, R. E., Dimarchi, R. D., Hoffmann, J. A., Long, H. B., & Miller, A. R. (n.d.).

Hua, Q. X., Nakagawa, S. H., Jia, W., Huang, K., Phillips, N. B., Hu, S. Q., & Weiss, M. A. (2008). Design of an active ultrastable single-chain insulin analog: synthesis, structure, and therapeutic implications. The Journal of biological chemistry, 283(21), 14703–14716. doi:10.1074/jbc.M800313200