Composite

Part:BBa_K3003018

Designed by: Ali Göktuğ Attar   Group: iGEM19_Bilkent-UNAMBG   (2019-10-16)


TEV Protease in Ag43 Cassette

Tobacco etch virus (TEV) protease is a site-specific protease. In this part, we placed TEV protease in Ag43 cassette to display it on the cell surface. This part can be used to cleave other peptides displayed on the cell surface who has specific TEV recognition site. TEV protease was expressed with low, mid and high copy level to characterize how it cuts the protein of interest from the specific site. System was characterized by the growth curve of the cell and under an inducible promoter, how protein of interest is cut was shown.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 280
    Illegal PstI site found at 1021
    Illegal PstI site found at 1579
    Illegal PstI site found at 2179
    Illegal PstI site found at 2803
    Illegal PstI site found at 3074
    Illegal PstI site found at 3127
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 280
    Illegal PstI site found at 1021
    Illegal PstI site found at 1579
    Illegal PstI site found at 2179
    Illegal PstI site found at 2803
    Illegal PstI site found at 3074
    Illegal PstI site found at 3127
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 280
    Illegal PstI site found at 1021
    Illegal PstI site found at 1579
    Illegal PstI site found at 2179
    Illegal PstI site found at 2803
    Illegal PstI site found at 3074
    Illegal PstI site found at 3127
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 280
    Illegal PstI site found at 1021
    Illegal PstI site found at 1579
    Illegal PstI site found at 2179
    Illegal PstI site found at 2803
    Illegal PstI site found at 3074
    Illegal PstI site found at 3127
    Illegal NgoMIV site found at 54
    Illegal NgoMIV site found at 847
    Illegal NgoMIV site found at 2071
    Illegal NgoMIV site found at 2581
    Illegal NgoMIV site found at 2602
    Illegal AgeI site found at 1597
    Illegal AgeI site found at 2341
    Illegal AgeI site found at 2755
    Illegal AgeI site found at 2997
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None