Composite

Part:BBa_K3003000:Design

Designed by: Ömer Can Ergül   Group: iGEM19_Bilkent-UNAMBG   (2019-10-16)


PelB-PenetraMax-SCI 57-SCI 57 Linker-Ag43


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 628
    Illegal PstI site found at 1186
    Illegal PstI site found at 1786
    Illegal PstI site found at 2410
    Illegal PstI site found at 2681
    Illegal PstI site found at 2734
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 628
    Illegal PstI site found at 1186
    Illegal PstI site found at 1786
    Illegal PstI site found at 2410
    Illegal PstI site found at 2681
    Illegal PstI site found at 2734
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 100
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 628
    Illegal PstI site found at 1186
    Illegal PstI site found at 1786
    Illegal PstI site found at 2410
    Illegal PstI site found at 2681
    Illegal PstI site found at 2734
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 628
    Illegal PstI site found at 1186
    Illegal PstI site found at 1786
    Illegal PstI site found at 2410
    Illegal PstI site found at 2681
    Illegal PstI site found at 2734
    Illegal NgoMIV site found at 54
    Illegal NgoMIV site found at 454
    Illegal NgoMIV site found at 1678
    Illegal NgoMIV site found at 2188
    Illegal NgoMIV site found at 2209
    Illegal AgeI site found at 1204
    Illegal AgeI site found at 1948
    Illegal AgeI site found at 2362
    Illegal AgeI site found at 2604
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None.


Source

Cloned from its components.

References

Hua, Q. X., Nakagawa, S. H., Jia, W., Huang, K., Phillips, N. B., Hu, S. Q., & Weiss, M. A. (2008). Design of an active ultrastable single-chain insulin analog: synthesis, structure, and therapeutic implications. The Journal of biological chemistry, 283(21), 14703–14716. doi:10.1074/jbc.M800313200