Composite

Part:BBa_K3002213:Design

Designed by: Dorothée Klein   Group: iGEM19_TU_Kaiserslautern   (2019-10-21)


L2 spectinomycin resistance + ARS_Mut-PETase_SP20HA + cCA_MHETase_SP20HA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5423
    Illegal PstI site found at 3491
    Illegal PstI site found at 4464
    Illegal PstI site found at 6765
    Illegal PstI site found at 7089
    Illegal PstI site found at 7432
    Illegal PstI site found at 8242
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5423
    Illegal NheI site found at 2665
    Illegal NheI site found at 5687
    Illegal PstI site found at 3491
    Illegal PstI site found at 4464
    Illegal PstI site found at 6765
    Illegal PstI site found at 7089
    Illegal PstI site found at 7432
    Illegal PstI site found at 8242
    Illegal NotI site found at 7100
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5423
    Illegal BglII site found at 8010
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5423
    Illegal PstI site found at 3491
    Illegal PstI site found at 4464
    Illegal PstI site found at 6765
    Illegal PstI site found at 7089
    Illegal PstI site found at 7432
    Illegal PstI site found at 8242
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5423
    Illegal PstI site found at 3491
    Illegal PstI site found at 4464
    Illegal PstI site found at 6765
    Illegal PstI site found at 7089
    Illegal PstI site found at 7432
    Illegal PstI site found at 8242
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1694
    Illegal NgoMIV site found at 3226
    Illegal NgoMIV site found at 3253
    Illegal NgoMIV site found at 5024
    Illegal NgoMIV site found at 6698
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.


Source

This part is a combination of the parts BBa_K3002102 (spectinomycin resistance), BBa_K3002111 (ARS_Mutant PETase_SP20HA) and BBa_K3002114 (cCA_wildtype MHETase_SP20HA).

References