Composite

Part:BBa_K3002112:Design

Designed by: Marlene Schlosser   Group: iGEM19_TU_Kaiserslautern   (2019-10-21)


Level 1 – MHETase + HA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1400
    Illegal PstI site found at 1724
    Illegal PstI site found at 2067
    Illegal PstI site found at 2877
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 264
    Illegal PstI site found at 1400
    Illegal PstI site found at 1724
    Illegal PstI site found at 2067
    Illegal PstI site found at 2877
    Illegal NotI site found at 1735
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2645
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1400
    Illegal PstI site found at 1724
    Illegal PstI site found at 2067
    Illegal PstI site found at 2877
    Illegal NgoMIV site found at 1333
    Illegal NgoMIV site found at 1794
    Illegal NgoMIV site found at 1824
    Illegal NgoMIV site found at 2139
    Illegal NgoMIV site found at 2157
    Illegal NgoMIV site found at 2193
    Illegal NgoMIV site found at 2836
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.



Source

This part is a combination of the parts BBa_K2516001 (PAR-promoter), BBa_K3002006 (RPL23-Terminator), BBa_K3002017 (HA-tag), BBa_K3002037 (CDS of Wildtype-MHETase), BBa_K3002303 (connector B2-B3), BBa_K3002304 (connector B4-B5) and BBa_K3002305 (connector B5-B6).

References