RNA
Part:BBa_K3001023:Design
Designed by: Sydnee Calhoun Group: iGEM19_Lethbridge_HS (2019-10-10)
Lsh crRNA targeting RNA Mango with T7 promoter
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 67
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 67
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 67
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 67
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part contains a T7 promoter to allow in vitro transcription with a T7 polymerase. A double terminator containing a native E. coli terminator and the T7 terminator is also present to signal transcription termination.
Source
This part was designed by Laura Keffer-Wilkes based on Munich 2017's designs. Synthesized by IDT.