RNA

Part:BBa_K3001022:Design

Designed by: Sydnee Calhoun, Laura Keffer-Wilkes   Group: iGEM19_Lethbridge_HS   (2019-10-10)


Lsh crRNA targeting RNA Mango


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 40
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 40
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 40
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 40
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Section of RNA Mango II that was targeted had to be specific to ensure that this was what would only be cleaved initially by Cas13a Lsh.

First segment of the sequence is a direct repeat stem loop to allow the crRNA to interact with Cas13a.

Source

Designed by Laura Keffer-Wilkes based off of designs by Munich 2017. Synthesized by IDT

References