RNA

Part:BBa_K3001016:Design

Designed by: Sydnee Calhoun   Group: iGEM19_Lethbridge_HS   (2019-10-10)


Lba crRNA targeting RNA Mango


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 40
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 40
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 40
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 40
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A direct stem loop is present to enable interactions between the crRNA and Cas13a Lba. The target sequence needs to be specific for just RNA Mango II to avoid off-target effects.


Source

Designed by Laura Keffer-Wilkes based on designs by Munich 2017. Synthesized by IDT.

References