RNA

Part:BBa_K3001014:Design

Designed by: Sydnee Calhoun   Group: iGEM19_Lethbridge_HS   (2019-10-12)


Lsh crRNA targeting GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A direct repeat stem loop was added to allow the crRNA to interact with Cas13a Lsh. The target sequence needs to be specific for GFP to avoid off-target effects.


Source

Designed by Laura Keffer-Wilkes based off of designs by Munich 2017. Synthesized by IDT.

References