Coding

Part:BBa_K3001002:Design

Designed by: Sydnee Calhoun   Group: iGEM19_Lethbridge_HS   (2019-10-14)


Cas13a Lbu Coding Sequence


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1068
    Illegal PstI site found at 1923
    Illegal PstI site found at 2862
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1068
    Illegal PstI site found at 1923
    Illegal PstI site found at 2862
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 349
    Illegal BglII site found at 1285
    Illegal BglII site found at 1495
    Illegal BglII site found at 1549
    Illegal BglII site found at 1780
    Illegal BglII site found at 2053
    Illegal BglII site found at 2539
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1068
    Illegal PstI site found at 1923
    Illegal PstI site found at 2862
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1068
    Illegal PstI site found at 1923
    Illegal PstI site found at 2862
    Illegal NgoMIV site found at 2001
    Illegal NgoMIV site found at 2637
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Need to overexpress in E. coli that contains rare codons.


Source

Ordered from Addgene

References