Device

Part:BBa_K300012:Experience

Designed by: Lorenzo Pasotti, Susanna Zucca, Nicolo' Politi   Group: iGEM10_UNIPV-Pavia   (2010-10-15)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K300012

User Reviews

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UNIPV-Pavia iGEM 2010


The parameters that summarize this part quantitative behaviour are reported in the table below. For details about the meaning of these parameters and the estimation methods, see BBa_K300010 Experience page.

Self-inducible device
working
not working 		LB M9
O.D.start K_HSL
[nmol/min] 		Doubling time
[min] 		Scell ratio O.D.start K_HSL
[nmol/min] 		Doubling time
[min] 		Scell ratio
BBa_K300012 (wiki name: I12) in pSB1A2 plasmid


Pv BBa K300012.png

0.50
±
0.01 		1.16 10^-17
±
6.41 10^-19 		36.66
±
2.50 		0.58
±
0.02 		0.30
±
0.03 		6.53 10^-17
±
1.43 10^-17 		50.92
±
2.92 		0.21
±
0.06


Absorbance, fluorescence and GFP synthesis rate signals of a typical experiment on this device, obtained as described in BBa_K300010 Experience page.

Growth curve of BBa_K300012 (O.D.600)
Fluorescence curve of BBa_K300012 (G.F.P.)
Fluorescence VS Optical density curve of BBa_K300012
Scell=(dGFP/dt)/O.D.600 of BBa_K300012 and threshold
;

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